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The circFTO levels in cataract patients, non-PDR and PDR patients (A) and HG treated HRECs (B) were determined with RT-qPCR. ***P<0.001 VS cataract group. &&&P<0.001 VS non-PDR group. (C) The genomic locus and sequence of circFTO. RT-qPCR was performed to detect the expressions of liner FTO and liner FTO after RNase R (D) and Actinomycin D (E) treatment. (A) The differences were detected using One-way ANOVA (n=30). (B, D, E) The differences were detected using Student-T test (n=3).

Journal: PLOS One

Article Title: Circular RNA FTO functions as a hsa-miR-141-3p sponge to regulate the growth and migration abilities of human retinal endothelial cells via up-regulating ZEB1

doi: 10.1371/journal.pone.0338208

Figure Lengend Snippet: The circFTO levels in cataract patients, non-PDR and PDR patients (A) and HG treated HRECs (B) were determined with RT-qPCR. ***P<0.001 VS cataract group. &&&P<0.001 VS non-PDR group. (C) The genomic locus and sequence of circFTO. RT-qPCR was performed to detect the expressions of liner FTO and liner FTO after RNase R (D) and Actinomycin D (E) treatment. (A) The differences were detected using One-way ANOVA (n=30). (B, D, E) The differences were detected using Student-T test (n=3).

Article Snippet: Human retinal microvascular endothelial cells (HRECs) were obtained from Innoprot® (P10880; Derio–Bizkaia, Spain).

Techniques: Quantitative RT-PCR, Sequencing

A-B: The target hsa-miR-141-3p and binding sites of circFTO was predicted by starbase software and validated by the dual-luciferase reporter. The hsa-miR-141-3p expression in HRECs was determined by RT-qPCR after si- circFTO (C) and HG (D) treatment. The differences were detected using Student-T test (n=3).

Journal: PLOS One

Article Title: Circular RNA FTO functions as a hsa-miR-141-3p sponge to regulate the growth and migration abilities of human retinal endothelial cells via up-regulating ZEB1

doi: 10.1371/journal.pone.0338208

Figure Lengend Snippet: A-B: The target hsa-miR-141-3p and binding sites of circFTO was predicted by starbase software and validated by the dual-luciferase reporter. The hsa-miR-141-3p expression in HRECs was determined by RT-qPCR after si- circFTO (C) and HG (D) treatment. The differences were detected using Student-T test (n=3).

Article Snippet: Human retinal microvascular endothelial cells (HRECs) were obtained from Innoprot® (P10880; Derio–Bizkaia, Spain).

Techniques: Binding Assay, Software, Luciferase, Expressing, Quantitative RT-PCR

A-B: The target hsa-miR-141-3p and binding sites of ZEB1 was confirmed by the targetscan database and validated by the dual-luciferase reporter gene system. The ZEB1 expressions in HRECs were measured with RT-qPCR after hsa-miR-141-3p mimic (C). The mRNA (D) and protein (E) levels of ZEB1 were detected by RT-qPCR and western blot in HG-treated and circFTO knockdown HRECs. (A-C) The differences were detected using Student-T test (n=3). (D) The differences were detected using One-way ANOVA (n=3).

Journal: PLOS One

Article Title: Circular RNA FTO functions as a hsa-miR-141-3p sponge to regulate the growth and migration abilities of human retinal endothelial cells via up-regulating ZEB1

doi: 10.1371/journal.pone.0338208

Figure Lengend Snippet: A-B: The target hsa-miR-141-3p and binding sites of ZEB1 was confirmed by the targetscan database and validated by the dual-luciferase reporter gene system. The ZEB1 expressions in HRECs were measured with RT-qPCR after hsa-miR-141-3p mimic (C). The mRNA (D) and protein (E) levels of ZEB1 were detected by RT-qPCR and western blot in HG-treated and circFTO knockdown HRECs. (A-C) The differences were detected using Student-T test (n=3). (D) The differences were detected using One-way ANOVA (n=3).

Article Snippet: Human retinal microvascular endothelial cells (HRECs) were obtained from Innoprot® (P10880; Derio–Bizkaia, Spain).

Techniques: Binding Assay, Luciferase, Quantitative RT-PCR, Western Blot, Knockdown